Journal: Journal of Functional Foods

Article Title: Anti-inflammatory effects and molecular mechanisms of loquat (Eriobotrya japonica) tea

doi: 10.1016/j.jff.2013.11.019

Figure Lengend Snippet: Fig. 5 – Inhibition of C and C2 fractions on NF-jB signaling. The cells were treated by C (A, C) and C2 (B, D) fractions of LTE in indicated concentrations for 30 min and then stimulated with LPS (40 ng/ml) for 30 min to detect I-jB or with LPS (1 lg/ml) for 10 min to detect IKK and TAK1, or 3 h to detect IRF3 and its phosphorylation by Western blotting with their antibodies respectively. The induction fold of proteins was calculated as the intensity of the treatment relative to that of control by densitometry. The blots shown are the examples of two separated experiments.

Article Snippet: Antibodies against phosphor-ERK1/2, phosphor-p38 kinase, phosphor-JNK, ERK1/2, p38 kinase, JNK, phosphor-TAK1 (Thr184/187), phospho-IKKa/b (ser176/180), IjB-a and phospho-IRF3 were purchased from Cell Signaling Technology, Beverly, MA, USA.

Techniques: Inhibition, Phospho-proteomics, Western Blot, Control

Journal: Journal of Traditional Chinese Medical Sciences

Article Title: Exploring the mechanism of Yishen Daluo decoction in the treatment of multiple sclerosis based on network pharmacology and in vitro experiments

doi: 10.1016/j.jtcms.2023.03.002

Figure Lengend Snippet: Fig. 6. Effect of YSDLD on the expression of (A) AKT & p-AKT (B) PI3K & p-PI3K using Western blot analysis. Notes: YSDLD: Yishen Daluo decoction; LPS: lipopolysaccharide; p-AKT: phosphorylation-protein kinase B; AKT: protein kinase B; p-PI3K: phosphorylation-phosphatidylinositol3- kinase; PI3K: phosphatidylinositol3-kinase. #P < .05 vs. the control group; *P < .05 vs. the LPS group.

Article Snippet: At 4 C, the membrane was incubated with primary antibodies overnight (1:1000; rabbit antibodies to phosphorylated (p)-Akt, Akt, phosphorylated (p)-PI3K, PI3K; Cell Signaling Technology, Beverly, MA).

Techniques: Expressing, Western Blot, Phospho-proteomics, Control